Stimuli-responsive polypeptide nanogels for trypsin inhibition

• Petr Šálek,
• Jana Dvořáková,
• Sviatoslav Hladysh,
• Diana Oleshchuk,
• Ewa Pavlova,
• Jan Kučka and
• Vladimír Proks

Beilstein J. Nanotechnol. 2022, 13, 538–548, doi:10.3762/bjnano.13.45

• inflammatory mediator trypsin. Two types of nanogels were prepared from linear synthetic polypeptides based on biocompatible and biodegradable poly[N5-(2-hydroxyethyl)-ʟ-glutamine-ran-N5-propargyl-ʟ-glutamine-ran-N5-(6-aminohexyl)-ʟ-glutamine]-ran-N5-[2-(4-hydroxyphenyl)ethyl)-ʟ-glutamine] (PHEG-Tyr) or

• trypsin because they contain suitable amino acids in their structures that effectively block the active site of trypsin. Keywords: α1-antitrypsin; inflammatory mediator; nanogel; polypeptide; trypsin; Introduction Despite significant and progressive advances in medicine, the global incidence of

• demonstrate stimuli-responsive behavior. These properties and their release behavior of AAT makes them successful candidates for prompt inhibition of the inflammatory mediator trypsin. Pilot in vitro study of trypsin inhibition As a next step, the inhibition activity of PHEG-Tyr and Nα-Lys-NG nanogels loaded